Producing hundreds of proteins for simultaneous kinetic analysis is prohibitively expensive and typically requires expression and purification of each protein individually in the lab in large batches, or the purchase of commercially expressed protein at hundreds of dollars each for the smallest available quantity.
In addition, the analyte of interest can be especially costly if it can only be used for analysis against a single ligand per run. The ability to use an analyte once against hundreds of proteins simultaneously would thus significantly decrease costs of interaction and kinetic analysis.
INanoBio has addressed these issues with cost by leveraging slides etched with thousands of nano-liter volume wells (nanowells). Each nanowell can be thought of as a nanoscale expression vessel, in which:
(1) a plasmid containing a unique gene of interest + a gene for a capture tag is printed,
(2) human lysate for cell-free expression is added,
(3) the tagged protein is expressed and
(4) immediately captured in the same pattern as the nanowell slide onto a gold biosensor slide (see image).
This process results in a low cost, single step arraying of hundreds to thousands of unique proteins onto a biosensor slide which can be used immediately for kinetic/interaction analysis or stored until needed