SPOC Overview

Measuring protein kinetics at scale

SPOC technology is the world’s first highly multiplexed proteomic biosensor. We developed technology that enables at least 1000 unique proteins to be simultaneously produced and capture-purified directly onto a 1.5 cm2 gold coated biosensor chip for surface plasmon resonance (SPR) analysis. Our biosensors are produced at the time of need, preserving protein structure and functionality to the fullest extent, and can also be stored for at least 10 days without loss in protein function. Requiring only the DNA sequence of interest, our unique production method enables us to readily provide custom chips to our clients with almost any protein sequence of interest.

Our core technology

Our core technology is our proprietary SPOC biosensor chip production method, which enables high-density protein arrays to be produced directly on the biosensor chip surface.

  1. First, we print DNA plasmids into individual nanowells. Each plasmid encodes a single protein fused to a common capture tag.
  2. Human cell-free lysate is added to initiate protein expression. Use of human lysate enables more native protein folding, proper functionality, and post-translational modifications (PTMs).
  3. The SPR biosensor chip is incubated in a sealed chamber with the nanowell slide. This prevents diffusion and cross-contamination between nanowells expressing unique proteins.
  4. As proteins are expressed, the co-expressed fusion capture tag enables simultaneous capture-purification directly onto the biosensor surface, which has been coated with a ligand that binds to the capture tag. This allows for specific capture of proteins of interest on biosensor surface, increasing specificity of our assay without the need to design production and purification pipelines for thousands of recombinant proteins – effectively reducing cost 10-100x from traditional workflows.
  5. After 2 to 4 hours of expression and capture, the biosensor is separated from nanowells slide, to display discrete, purified protein spots that are ready for SPR kinetic measurements.


When the SPOC chip is docked to an SPR instrument, it allows for simultaneous data analysis from thousands of proteins. A sample (or analyte) is injected and binding interactions are measured on the sensor surface, providing qualitative, quantitative, and kinetic data.

Our arrays were rigorously validated to have no cross-talk with reproducible kinetic data, even with 1000 proteins per chip. Antigen-specific antibodies to p53, Jun, and Src were measured by SPR and found to only bind to the protein spots specific to that antibody target. This step is critical to the accuracy of SPR data and our production method was iteratively optimized to ensure consistency in protein expression and capture at discrete locations on SPOC chips, so you can trust your data is for the intended protein.

Primary chip validation

Our primary chip validation was performed on a Carterra LSAXT instrument, and we also have chips available for the Horiba OpenPleX. Each system has a unique set-up and throughput (see figure below). If you work with a different platform, contact us to inquire about compatibility – we’d be happy to work with you.