SPOC technology is the world’s first highly multiplexed proteomic biosensor. We developed technology that enables at least 1000 unique proteins to be simultaneously produced and capture-purified directly onto a 1.5 cm2 gold coated biosensor chip for surface plasmon resonance (SPR) analysis. Our biosensors are produced at the time of need, preserving protein structure and functionality to the fullest extent, and can also be stored for at least 10 days without loss in protein function. Requiring only the DNA sequence of interest, our unique production method enables us to readily provide custom chips to our clients with almost any protein sequence of interest.
Our core technology is our proprietary SPOC biosensor chip production method, which enables high-density protein arrays to be produced directly on the biosensor chip surface.
Our arrays were rigorously validated to have no cross-talk with reproducible kinetic data, even with 1000 proteins per chip. Antigen-specific antibodies to p53, Jun, and Src were measured by SPR and found to only bind to the protein spots specific to that antibody target. This step is critical to the accuracy of SPR data and our production method was iteratively optimized to ensure consistency in protein expression and capture at discrete locations on SPOC chips, so you can trust your data is for the intended protein.
Our primary chip validation was performed on a Carterra LSAXT instrument, and we also have chips available for the Horiba OpenPleX. Each system has a unique set-up and throughput (see figure below). If you work with a different platform, contact us to inquire about compatibility – we’d be happy to work with you.