Our core technology is our proprietary SPOC biosensor chip production method, which enables high-density protein arrays to be produced directly on the biosensor chip surface.

1. First, we print DNA plasmids into individual nanowells. Each plasmid encodes a single protein fused to a common capture tag.
2. Human cell-free lysate is added to initiate protein expression. Use of human lysate enables more native protein folding, proper functionality, and post-translational modifications (PTMs).
3. The SPR biosensor chip is incubated in a sealed chamber with the nanowell slide. This prevents diffusion and cross-contamination between nanowells expressing unique proteins.
4. As proteins are expressed, the co-expressed fusion capture tag enables simultaneous capture-purification directly onto the biosensor surface, which has been coated with a ligand that binds to the capture tag. This allows for specific capture of proteins of interest on biosensor surface, increasing specificity of our assay without the need to design production and purification pipelines for thousands of recombinant proteins – effectively reducing cost 10-100x from traditional workflows.
5. After 2 to 4 hours of expression and capture, the biosensor is separated from nanowells slide, to display discrete, purified protein spots that are ready for SPR kinetic measurements.